کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8347901 | 1541708 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hyperglycemic activity of the recombinant crustacean hyperglycemic hormone B1 isoform (CHH-B1) of the Pacific white shrimp Litopenaeus vannamei
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کلمات کلیدی
PBSRP-HPLCTFATCAPAMTrifluoroacetic acid - اسید Trifluoroacetictrichloroacetic acid - اسید ترشکلراکتیکSodium dodecylsulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل سدیم دودسیل سولفات - پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدphosphate saline buffer - بافر فسفات نمکRecombinant expression - بیان ترکیبیLitopenaeus vannamei - میگوی سفید غربی، لیتوپنئوس وانامیpoint accepted mutation - نقطه جهش پذیرفته شده استCrustacean Hyperglycemic Hormone - هورمون هیپرگلیسمی Crustaceanpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازReverse-phase high performance liquid chromatography - کروماتوگرافی مایع با کارایی معکوس فاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Crustacean hyperglycemic hormone (CHH) is the most abundant neuropeptide produced by the X-organ/sinus gland (XO/SG) complex in the crustacean eyestalk. CHH plays a principal role in the control of glucose metabolism. The CHH-B1 isoform is produced in the eyestalk of Litopenaeus vannamei by alternative splicing of the chhB gene and its cDNA sequence has revealed that this isoform has a non-amidated C-terminal residue (CHH-like peptide). In this work, a recombinant CHH-B1 (rCHH-B1) with a sequence identical to the native hormone was expressed in the methylotrophic yeast Pichia pastoris X-33 and purified from the culture medium by RP-HPLC. The identity of the purified rCHH-B1 was confirmed by N-terminal sequencing and by using an anti-CHH-B1 polyclonal antibody. An in vivo assay showed that the hyperglycemic effect was dependant of the dosage of rCHH-B1, and the maximal hyperglycemic response was obtained with 250Â pmol treatment. These results suggest that the amino acid sequence of the C-terminus and its correct structure are both important for the hyperglycemic activity of naturally occurring non-amidated CHH peptides, such as CHH-B1. CHH-B1 appears to be the first reported CHH-like peptide with significant hyperglycemic activity produced in the sinus gland of a penaeid shrimp.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 71, September 2015, Pages 32-39
Journal: Peptides - Volume 71, September 2015, Pages 32-39
نویسندگان
Laura Camacho-Jiménez, Edna Sánchez-Castrejón, Elizabeth Ponce-Rivas, Ma. Enriqueta Muñoz-Márquez, Manuel B. Aguilar, Ana Denisse Re, Fernando DÃaz,