کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8348296 | 1541720 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of novel dipeptidyl peptidase-IV and angiotensin-I-converting enzyme inhibitory peptides from meat proteins using in silico analysis
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کلمات کلیدی
GIPDPP-IVRP-HPLCACE-IAmCHBARAASGLP-1T2DIEPHBPHBBMYH2MALDI-TOFACTSDMSO - DMSOSerum albumin - آلبومین سرم aminomethylcoumarin - آمینو متیل کومارینangiotensin-I-converting enzyme - آنزیم آنژیوتانسین I تبدیل آنBasic Local Alignment Search Tool - ابزار جستجوی محلی سازگاری محلیBlast - انفجارQSAR - بزرگسال cardiovascular disease - بیماری قلب و عروقیIn silico analysis - در تجزیه و تحلیل سیلیکاGastrointestinal - دستگاه گوارشType-2 diabetes - دیابت نوع 2dipeptidyl peptidase-IV - دیپپتیدیل پپتیداز IVDimethylsulfoxide - دیمتیل سولفواکسیدCVD - رسوب دهی شیمیایی بخار Metabolic syndrome - سندرم متابولیکrenin–angiotensin–aldosterone system - سیستم رنین-آنژیوتانسین-آلدوسترونHigh blood pressure - فشار خون بالاSupport vector machine - ماشین بردار پشتیبانیSVM - ماشین بردار پشتیبانیMETS - متسmatrix-assisted laser desorption/ionization-time of flight - مدت زمان جذب / زمان یونیزاسیون لیزر ماتریس کمک می کندMyoglobin - میوگلوبینIsoelectric point - نقطه ایزوالکتریکMolecular weight - وزن مولکولیUnited Kingdom - پادشاهی متحده بریتانیاMeat proteins - پروتئین گوشتglucagon-like peptide-1 - پپتید 1-گلوکاگون-مانندGastric inhibitory peptide - پپتید مهار کننده معده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of novel dipeptidyl peptidase-IV and angiotensin-I-converting enzyme inhibitory peptides from meat proteins using in silico analysis Identification of novel dipeptidyl peptidase-IV and angiotensin-I-converting enzyme inhibitory peptides from meat proteins using in silico analysis](/preview/png/8348296.png)
چکیده انگلیسی
Angiotensin-I-converting enzyme (ACE-I, EC 3.4.15.1), renin (EC 3.4.23.15), and dipeptidyl peptidase-IV (DPP-IV, EC 3.4.14.5) play key roles in the control of hypertension and the development of type-2 diabetes and other diseases associated with metabolic syndrome. The aim of this work was to utilize known in silico methodologies, peptide databases and software including ProtParam (http://web.expasy.org/protparam/), Basic Local Alignment Tool (BLAST), ExPASy PeptideCutter (http://web.expasy.org/peptide_cutter/) and BIOPEP (http://www.uwm.edu.pl/biochemia/index.php/pl/biopep) to assess the release of potentially bioactive DPP-IV, renin and ACE-I inhibitory peptides from bovine and porcine meat proteins including hemoglobin, collagen and serum albumin. These proteins were chosen as they are found commonly in meat by-products such as bone, blood and low-value meat cuts. In addition, the bioactivities of identified peptides were confirmed using chemical synthesis and in vitro bioassays. The concentration of peptide required to inhibit the activity of ACE-I and DPP-IV by 50% was determined for selected, active peptides. Novel ACE-I and DPP-IV inhibitory peptides were identified in this study using both in silico analysis and a literature search to streamline enzyme selection for peptide production. These novel peptides included the ACE-I inhibitory tri-peptide Ile-Ile-Tyr and the DPP-IV inhibitory tri-peptide Pro-Pro-Leu corresponding to sequences f (182-184) and f (326-328) of both porcine and bovine serum albumin which can be released following hydrolysis with the enzymes papain and pepsin, respectively. This work demonstrates that meat proteins are a suitable resource for the generation of bioactive peptides and further demonstrates the usefulness of in silico methodologies to streamline identification and generation of bioactive peptides.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 59, September 2014, Pages 53-62
Journal: Peptides - Volume 59, September 2014, Pages 53-62
نویسندگان
Tomas Lafarga, Paula O'Connor, Maria Hayes,