کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8383248 | 1543292 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Telomere repeats and macronuclear DNA organization in the soil ciliate Kahliella matisi (Ciliophora, Hypotricha)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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چکیده انگلیسی
To better understand the structure of macronuclear chromosomes in ciliates, the organization of macronuclear DNA was investigated in the hypotrich Kahliella matisi. Total DNA of K. matisi separated by agarose gel electrophoresis showed continuous smear ranging in size from â¼500Â bp to â¼15Â kb. This fragmentation was found to be due to the presence of gene-sized macronuclear chromosomes. The sequence analysis of four randomly cloned macronuclear chromosomes showed that K. matisi telomeres consist of 5â²-dC4A4-3â² repeats and carry one or two open reading frames. The transcription unit was found to be flanked with non-coding AT rich 5â² leader and 3â² trailer. No consensus transcription-regulatory sequences were identified in 5â² leader and only one of analyzed gene-sized chromosomes showed the presence of conserved poly(A) addition signal sequence in 3â² trailer. All ORFs showed highest relatedness to Oxytricha trifallax macronuclear chromosomes with conserved exon/intron structure. Sequence comparisons indicate that macronuclear chromosome organization is at least partially conserved in ciliates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Protistology - Volume 50, Issue 3, June 2014, Pages 231-235
Journal: European Journal of Protistology - Volume 50, Issue 3, June 2014, Pages 231-235
نویسندگان
TÃmea Å paková, Peter PristaÅ¡, Peter Javorský,