کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8383321 | 1543296 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ciliate-adenovirus interactions in experimental co-cultures of Euplotes octocarinatus and in wastewater environment
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Ciliate-adenovirus interactions in experimental co-cultures of Euplotes octocarinatus and in wastewater environment Ciliate-adenovirus interactions in experimental co-cultures of Euplotes octocarinatus and in wastewater environment](/preview/png/8383321.png)
چکیده انگلیسی
The aim of the present work was to study the dynamics of the interactions between human adenovirus and ciliates under both experimental and field conditions. Experimental co-cultures of the ciliated protozoan Euplotes octocarinatus and human adenovirus (HAdV) type 2 were established and virus internalization was investigated using nested PCR and direct immunofluorescence (IF). In addition, to study protozoa-virus interactions in the field, wild ciliates were isolated from active sludges of a wastewater treatment plant and analyzed for the presence of adenovirus using direct IF. In vitro experiments revealed HAdV type 2 inside Euplotes cells after 15Â min of contact and its persistence until at least 35 days post infection. In addition, our results showed the adsorption of adenovirus on the surface of wild ciliates. We conclude that HAdV is taken up by ciliates, however more studies are necessary in order to better investigate the mechanisms, the infectivity of internalized virus and the protective effects of internalization against disinfection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Protistology - Volume 49, Issue 3, August 2013, Pages 381-388
Journal: European Journal of Protistology - Volume 49, Issue 3, August 2013, Pages 381-388
نویسندگان
Roberta Battistini, Elisa Marcucci, Marco Verani, Graziano Di Giuseppe, Fernando Dini, Annalaura Carducci,