کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8392222 | 1544005 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Capsule gene CAP64 is involved in the regulation of vacuole acidification in Cryptococcus neoformans
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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چکیده انگلیسی
A basidiomycetous yeast Cryptococcus neoformans is stained by DBB. We found that the edges of DBB stained cells were specifically detected by using fluorescence microscopy. We also found that the only the edges of cap64Î strain cells was not fluorescent among several acapsular mutants, although whose colonies turned to red or light pink by DBB staining. When the vacuoles were stained by FM4-64, those of the cap64Î cells showed aberrant morphology. In addition, quinacrine treatment showed that the cap64Î strain could not accumulate quinacrine in the vacuole. These data suggest that Cap64 was not only involved in capsule formation, but also in intracellular pH regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mycoscience - Volume 58, Issue 1, January 2017, Pages 45-52
Journal: Mycoscience - Volume 58, Issue 1, January 2017, Pages 45-52
نویسندگان
Yumi Imanishi, Reiko Tanaka, Takashi Yaguchi, Kiminori Shimizu,