کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8401838 | 1544494 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Voltage-gated Na+ channel transcript patterns in the mammalian heart are species-dependent
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
TTXSUDEPNRSF/RESTGene expression - بیان ژنtetrodotoxin - تترو دوتوکسین Alternative splicing - جابجایی جایگزینReal-time RT-PCR - زمان واقعی RT-PCRReal-time reverse transcriptase-polymerase chain reaction - زمان واقعی واکنش زنجیره ای ترانس کریتاز - پلیمراز معکوسCardiomyocytes - قلب و عروقSudden unexpected death in epilepsy - مرگ ناگهانی غیرمنتظره در صرعDevelopmental regulation - مقررات توسعهNa+ channels - کانالهای Na +
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the present study we investigated whether transcription, alternative splicing and developmental regulation of voltage-gated Na+ channels occur in a species-dependent manner in the mammalian heart. The composition of the Na+ channel transcript pool including Nav1.1-Nav1.5 was analysed by RT-PCR in mouse, rat, pig, and human hearts. We found that relative transcript levels of tetrodotoxin (TTX) sensitive channels (Nav1.1-Nav1.4) decreased with increasing heart size (30% for mouse, 8% for rat, and 4% for pig/human). Considering transcript levels of individual isoforms, human and pig hearts were nearly indistinguishable whereas large differences existed between human and mouse. We also noticed that alternative splicing and age-dependent Na+ channel expression occurred in a species-dependent manner. Unexpectedly, we even observed gender differences in the cardiac levels of TTX sensitive Na+ channels in humans. Our data suggest that species differences could underlie published discrepancies on the role of TTX sensitive Na+ channels in the heart and that the functions of those minor cardiac isoforms in normal and diseased hearts are best studied in larger mammalian animals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Progress in Biophysics and Molecular Biology - Volume 98, Issues 2â3, OctoberâNovember 2008, Pages 309-318
Journal: Progress in Biophysics and Molecular Biology - Volume 98, Issues 2â3, OctoberâNovember 2008, Pages 309-318
نویسندگان
Steve Blechschmidt, Volker Haufe, Klaus Benndorf, Thomas Zimmer,