کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8403769 1544682 2018 19 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Single layer centrifugation improves the quality of frozen-thawed sperm of giant panda (Ailuropoda melanoleuca)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Single layer centrifugation improves the quality of frozen-thawed sperm of giant panda (Ailuropoda melanoleuca)
چکیده انگلیسی
The reproductive capacity of captive giant pandas is poor and sperm cryopreservation is necessary for the reproduction and conservation of this species. Cryopreservation, however, leads to a significant decrease in sperm quality, including sperm motility, acrosome integrity and DNA integrity. In the present study, a method was developed based on colloid single layer centrifugation that could significantly improve frozen-thawed sperm quality. Two colloids were compared for post-thaw giant panda sperm preparation; the sperm samples had greater total motility (Colloid 1: 44.5 ± 16.0%, Colloid 2: 42.4 ± 10.1% compared with Control: 25.4 ± 8.4%, P < 0.05), linear velocity (Colloid 1: 17.2 ± 8.3 μm/s; Colloid 2: 19.0 ± 9.0 μm/s compared with Control: 6.6 ± 1.7 μm/s, P < 0.05) and membrane integrity (Colloid: 46.9 ± 13.2%; Colloid 2: 54.3 ± 5.7% compared with Control: 36.0 ± 9.1%; P < 0.05). This method could be a useful tool to enable the use of poor quality sperm samples and benefit this population by using available genetic material.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 195, August 2018, Pages 58-64
نویسندگان
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