کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8403817 | 1544684 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Geranylgeranylacetone induction of HSP90α exerts cryoprotective effect on Acipenser sinensis sperm
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Heat Shock Protein 90 (HSP90) is a fertility-associated protein, the expression of which positively correlates with sperm quality in many species. Geranylgeranylacetone (GGA) is reported to induce expression of HSP90. The present study aimed to investigate whether GGA induced expression of HSP90 in Acipenser sinensis sperm to exert a cryoprotective effect. Sperm from five male A. sinensis was combined with extender containing 20â¯mmol/L tris pHâ¯=â¯8.1, 10% v/v methanol, 2-5â¯mmol/L KCl, 15â¯mmol/L lactose, and 15â¯mmol/L trehalose, with GGA at 0, 14, 67, 135, 673, 1346, or 6731â¯Î¼mol/L. After cryopreservation and thawing, the percentage of motile spermatozoa, spermatozoon curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), acrosome integrity, and membrane integrity, as well as fertility were evaluated. Sperm quality increased with the increase of GGA to 673â¯Î¼mol/L, but decreased at higher concentrations. Expression levels of HSP90α were detected by Western blot in sperm frozen with GGA at 673â¯Î¼mol/L (highest obtained sperm quality), 6731â¯Î¼mol/L (highest GGA concentration), and a control without GGA. The expression of HSP90α increased with the increase in GGA, with lowest expression observed in the control. GGA was found to induce increase of HSP90α, and this increase was associated with higher quality cryopreserved sperm at concentrations â¤673â¯Î¼mol/L. This research suggests a viable technique to increase the quality of cryopreserved A. sinensis sperm by adding GGA to induce expression of HSP90α.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 193, June 2018, Pages 19-25
Journal: Animal Reproduction Science - Volume 193, June 2018, Pages 19-25
نویسندگان
Meng Dan Xi, Ping Li, Hao Du, Xin Mei Qiao, Zhi Gang Liu, Wei Qi Wei,