کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8403949 | 1544686 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Epididymal sperm from Spix's yellow-toothed cavies sperm successfully cryopreserved in Tris extender with 6% glycerol and 20% egg yolk
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
As a non-threatened hystricognath rodent species, Spix's yellow-toothed cavies can be used as a model for the development of assisted reproductive techniques for the conservation of closely related species. The objective was to establish a functional protocol for cryopreservation of epididymal sperm from these cavies. Twelve sexually mature males, â¼2â¯y old and weighing â¼300â¯g, were euthanized. Sperm were recovered by retrograde flushing of the vas deferens and cauda epididymis with Tris extender. Thereafter, sperm were extended in Tris plus 20% egg yolk, with 3%, 6% or 9% glycerol or dimethyl sulfoxide (DMSO), placed in 0.25â¯mL straws and cryopreserved in liquid nitrogen. Sperm concentration, motility (using computer-assisted sperm analysis; CASA), plasma membrane integrity, osmotic response, morphology and sperm binding-ability were determined in fresh and frozen-thawed sperm. For most sperm endpoints, glycerol was a more desirable cryoprotectant than DMSO. Data (meanâ¯Â±â¯SEM) were similar with use of 3%, 6%, and 9% glycerol (Pâ¯>â¯0.05) in osmotic response (40.66â¯Â±â¯6.3%, 42.5â¯Â±â¯7.1%, and 39.5â¯Â±â¯5.0% respectably), and membrane integrity (55.17â¯Â±â¯5.5%, 68.4â¯Â±â¯4.1%, and 59.1â¯Â±â¯4.9% respectably). Among concentrations assessed, the use of 6% glycerol resulted in the greatest (Pâ¯<â¯0.05) post-thaw values for total motility (60.9â¯Â±â¯4.4%), rapid subpopulation motility (27.7â¯Â±â¯3.1%) and sperm-binding capability (227.0â¯Â±â¯20.2). In conclusion, epididymal sperm from the Spix's yellow-toothed cavies (G. spixii) are optimally cryopreserved in Tris extender with 6% glycerol and 20% egg yolk.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 191, April 2018, Pages 64-69
Journal: Animal Reproduction Science - Volume 191, April 2018, Pages 64-69
نویسندگان
Andréia M. Silva, Erica C.G. Praxedes, LÃvia B. Campos, Luana G.P. Bezerra, Samara S.J. Moreira, Keilla M. Maia, Ana L.P. Souza, Alexandre R. Silva,