کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8404071 | 1544688 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cryoprotective effect of glutamine, taurine, and proline on post-thaw semen quality and DNA integrity of donkey spermatozoa
ترجمه فارسی عنوان
اثر محافظتی گلوتامین، تاورین و پرولین بر روی کیفیت منی و پس از خنثی سازی اسپرماتوسیا
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کلمات کلیدی
آمینو اسید، خرگوش اندلس، منجمد-منجمد منی، کیفیت اسپرم،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
This study was conducted to evaluate the effect of amino acid addition to semen on post-thaw quality of donkey spermatozoa. Eighteen ejaculates were pooled and divided into aliquots which were cryopreserved in Gent A® containing 1% ethylene glycol (Gent-EG) and supplemented with 0 (as control), 20, 40, or 60â¯mM of glutamine, proline, or taurine. The greatest concentration (60â¯mM) of glutamine and taurine resulted in greater (Pâ¯<â¯0.001) post-thaw sperm motility. Amino acid supplementation did not improve (Pâ¯>â¯0.05) sperm morphology and membrane plasma integrity compared with the control samples. Whereas, improvement (Pâ¯<â¯0.05) of acrosome integrity was observed with use of 60â¯mM glutamine. After thawing, there were no differences (Pâ¯>â¯0.05) in the sperm DNA fragmentation index (sDFI) among treatments. The 60â¯mM glutamine and 40â¯mM taurine treatments, however, resulted in a reduction (Pâ¯<â¯0.05) in sDFI values in the first 6â¯h of semen incubation, compared with the control samples. At 24â¯h, the sDFI values were less (Pâ¯<â¯0.05) in all supplemented as compared with control samples, except for the 20â¯mM proline treatment group. In conclusion, supplementation of the Gent-EG extender with glutamine or taurine at 60â¯mM improved post-thaw donkey sperm quality. The addition of proline to the freezing extender, however, did not provide any significant enhancement in sperm quality, compared with the control group.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 189, February 2018, Pages 128-135
Journal: Animal Reproduction Science - Volume 189, February 2018, Pages 128-135
نویسندگان
M. Bottrel, D. Acha, I. Ortiz, M. Hidalgo, J. Gósalvez, J. Camisão, J. Dorado,