کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8405813 | 1544897 | 2018 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A cold tolerant lipase develops enhanced activity, thermal tolerance and solvent stability in the presence of calcium nanoparticles: An alternative approach to genetic modulation
ترجمه فارسی عنوان
لیپاز متحمل به سرما باعث افزایش فعالیت، تحمل حرارتی و پایداری حلال در حضور نانوذرات کلسیم می شود: رویکرد جایگزین به مدولاسیون ژنتیکی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
An extracellular cold active lipase producing bacterium was isolated from soil. It was identified as Pseudomonas aeruginosa KC1 strain (GenBank accession number KT334371). There are no previous reports on purification of cold active lipase protein from Pseudomonas aeruginosa. The lipase of molecular weight 54â¯kDa was purified to 33 fold with 8% recovery. The enzyme was active within the range 10-40â¯Â°C with maximum activity at 15â¯Â°C; pH 7.0-8.5 with 4-nitrophenyl butyrate substrate. Substrate utilization by lipase showed better affinity for short chain fatty acid esters. The enzyme activity was enhanced by Ca2+, Ba2+, Fe2+ and Mg2+; inhibited by Hg2 +, Ni2+, Zn2+ and Co2+. Nano-calcium enabled KC1 lipase (NP-lip) showed enhanced activity for both short and long chain fatty acid esters (NP: 8.8â¯Âµg/mL) compared to CaCl2 (1â¯mM). The activity of the NP-lip system increased 72% at 15â¯Â°C and 7 fold at 55â¯Â°C while retaining activity for 4â¯h. Lowering of Km (55% at 15â¯Â°C; 45% at 55â¯Â°C) and increased Vmax (7 fold at 15â¯Â°C; 3.5 fold at 55â¯Â°C) was observed for NP-lip system. Heat deactivation kinetics for NP-lip showed drastic improvement in half-life at higher temperatures and entropy-enthalpy compensation. Furthermore the NP-lip system was stable in organic solvents and was effective in the esterification of butyl butyrate and trans-esterification of sunflower oil in n-hexane. This remarkable simultaneous enhancement of activity, temperature and organic solvent tolerance of the NP-lip has potential for industrial usage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 15, July 2018, Pages 1-10
Journal: Biocatalysis and Agricultural Biotechnology - Volume 15, July 2018, Pages 1-10
نویسندگان
Aniket Das, Krishanu Chakrabarti,