کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8405851 | 1544898 | 2018 | 48 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tissue-specific heat-induced changes in the expression of ncRNA fragments in Brassica rapa plants
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Tissue-specific heat-induced changes in the expression of ncRNA fragments in Brassica rapa plants Tissue-specific heat-induced changes in the expression of ncRNA fragments in Brassica rapa plants](/preview/png/8405851.png)
چکیده انگلیسی
Non-coding RNA fragments (ncRFs) represent a large spectrum of short ncRNAs processed from precursor ncRNAs such as tRNA, rRNA, snoRNA and several others. Several recent papers suggest that ncRFs are processed in a specific manner in different tissues or in response to stress. Here, we analyzed alterations in the expression of ncRFs in different Brassica rapa tissues in response to heat stress. Specifically, we analyzed the abundance of tRNA fragments (tRFs), rRNA fragments (rRFs), snoRNA fragments (snoRFs) and snRNA fragments (snRFs). The most significant changes in response to heat stress were observed in tRNAGlu and tRNAAsp. Whereas the number of reads mapping to the former one dropped drastically in all tissues but the apical meristem and pollen, the number of reads mapping to the latter significantly increased. Analysis of tRFs showed that three isoacceptors, tRF5â²Asp(GUC), tRFGly(UCC) and tRFPseudo(UCC) were severely underrepresented in heat-stressed tissues. Heat also induced changes in the processing of ncRFs from precursor molecules; a bias towards the processing of ncRFs from the 3â² end was even more prominent after the heat stress application. The analysis of predicted targets of ncRFs revealed an enrichment in several groups of genes involved in the response to various stresses. To conclude, our work showed that heat stress results in tissue-specific changes in the expression of ncRFs in Brassica rapa and suggested a tissue-specific regulation of various mRNAs and pathways in response to heat.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 14, April 2018, Pages 338-356
Journal: Biocatalysis and Agricultural Biotechnology - Volume 14, April 2018, Pages 338-356
نویسندگان
Boseon Byeon, Andriy Bilichak, Igor Kovalchuk,