کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8405922 | 1544897 | 2018 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation, identification and extraction of antimicrobial compounds produced by Streptomyces sps from terrestrial soil
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Isolation, identification and extraction of antimicrobial compounds produced by Streptomyces sps from terrestrial soil Isolation, identification and extraction of antimicrobial compounds produced by Streptomyces sps from terrestrial soil](/preview/png/8405922.png)
چکیده انگلیسی
The main objective of the present study was to find the antimicrobial compounds from terrestrial Strptomyces sps. Streptomycetes strains were isolated from terrestrial soil and subjected to primary screened by cross streak method against some pathogenic Gram-positive and Gram-negative test bacteria and fungi. Streptomycetes of which one isolate showing broad spectrum of antimicrobial was characterized on the basis of their morphological and physiological properties. The antimicrobial substances were extracted from active isolates and inoculated in ISP2 broth were fermented for 7 days at 28â¯Â°C by ethyl acetate extraction method. The active crude compounds were subjected to secondary screening by agar well diffusion method to confirm activity against same pathogenic bacteria and fungi. The crude compounds were purified by Thin layer chromatography (TLC). The bioactive region was detected on the TLC plate and calculated Rf value. Two bands were detected and each band tested for agar well diffusion method for antimicrobial activity. The characterization of the bioactive compounds was further determined by using UV-visible spectrophotometer, FT-IR and GC-MS techniques. These potent isolates from terrestrial soil could be an interesting source for pharmaceutical industries to explore antibacterial and antifungal compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 15, July 2018, Pages 317-321
Journal: Biocatalysis and Agricultural Biotechnology - Volume 15, July 2018, Pages 317-321
نویسندگان
S. Usha Nandhini, S. Sudha, V. Anusha Jeslin, S. Manisha,