Uptake and permeability studies of BBB-targeting immunoliposomes using the hCMEC/D3 cell line

Confocal microscopy of hCMEC/D3 monolayers formed on transwell membranes. Cells were treated with control liposomes (control), murine serum IgG-immunoliposomes (IgG), and OX-26 immunoliposomes (OX-26). All liposomes are stained with lipid-rhodamine (red) and encapsulate FITC (green). Nuclei are stained with DAPI (blue). Each column represents different slices obtained under the confocal microscope (3.75 μm/slice).