کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8416591 | 1545573 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biochemical and molecular analysis of Camellia sinensis (L.) O. Kuntze tea from the selected P/11/15 clone
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
Green tea is one of the most important beverages consumed across the world and it possesses various phytotherapeutics. Polyphenol oxidase (PPO) activity, total polyphenols, catechins, amino acid content and enzymatic antioxidants are considered to be potential parameters in tea characterization. P/11/15 clone (Camellia sinensis (L) O. Kuntze) was chosen to analyze the biochemical characterization and to analyze the gene expression pattern. The selected P/11/15 clone (C. sinensis (L) O. Kuntze) possess potent Polyphenol oxidase (49.62 U/mg of protein), sufficient catechin (20.75%), Polyphenol (20.01%), Peroxidase (450.08 μM of O2 formed minâ1 gâ1 dry weight), Catalase (1.20 μM H2O2 reduced minâ1 mgâ1 protein) and Super Oxide Dismutase (45.11 U/mg proteins). Flavonoid gene expression reveals ANR (1.66%) and F3H (1.02%) were up regulated in the selected P/11/15 clone. The results obtained suggest that P/11/15 clone showed adequate enzyme levels, thus an increased antioxidant activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Genetic Engineering and Biotechnology - Volume 14, Issue 1, June 2016, Pages 69-75
Journal: Journal of Genetic Engineering and Biotechnology - Volume 14, Issue 1, June 2016, Pages 69-75
نویسندگان
Samynathan Ramkumar, Perisamy Suresh kumar, Gandhi Sudhakar, J. Anitha, Sanmugam Geetha, Padmanabhan Mohankumar, Velliyur Kanniappan Gopalakrishnan,