کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8416605 | 1545573 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
Fusarium oxysporum which causes wilt is a serious pathogen. Fusarium isolates were isolated from Assam located in North East region of India. Morphological identification of Fusarium isolates was done using conidial and hyphal structures. Molecular identification of Fusarium isolates was done by amplifying the internal transcribed spacer (ITS) region of the conserved ribosomal DNA using primers ITS1 and ITS4. All the ITS sequences were compared for gaps and similarity. Further, characterization of random amplified polymorphic DNA (RAPD) was carried out using 40 primers. 15 primers that gave reproducible results were selected. RAPD was used to observe the relatedness among these isolates. Thus, it was concluded that molecular profiling using ITS is an indispensable method for identification studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Genetic Engineering and Biotechnology - Volume 14, Issue 1, June 2016, Pages 99-105
Journal: Journal of Genetic Engineering and Biotechnology - Volume 14, Issue 1, June 2016, Pages 99-105
نویسندگان
Irom Manoj Singha, Yelena Kakoty, Bala Gopalan Unni, Jayshree Das, Mohan Chandra Kalita,