کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8417609 1545693 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
TCR-mediated functions are enhanced in activated peripheral blood T cells isolated from leucocyte reduction systems
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
TCR-mediated functions are enhanced in activated peripheral blood T cells isolated from leucocyte reduction systems
چکیده انگلیسی
Buffy coats are the most common method for the acquisition of activated primary human T cells for research or clinical applications, but recently leukocyte reduction system (LRS) cones have emerged as a viable source for these cells. In this study, we determined if activated human T cells derived from buffy coats or LRS cones had different functionality. No changes in the expression of surface receptors were observed except for a significant increase in CD44 expression on T cells isolated from LRS cones. LRS cone-derived T cells trended towards higher receptor-mediated cytokine production and had significantly increased donor-to-donor variability in IFN-γ production. TCR-induced ERK1/ERK2 and AKT phosphorylation was also increased in T cells isolated from LRS cones. In conclusion, LRS cones are an excellent source of T cells for clinical and research applications, but these cells have subtle functional differences from T cells isolated using standard buffy coats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 416, January 2015, Pages 137-145
نویسندگان
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