کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8417638 | 1545695 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A method for high purity intestinal epithelial cell culture from adult human and murine tissues for the investigation of innate immune function
ترجمه فارسی عنوان
یک روش برای کشت سلول های اپیتلیال روده از بافت های بالغ انسانی و مغز برای بررسی عملکرد سیستم ایمنی ذاتی
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کلمات کلیدی
سلولهای اپیتلیال روده، فرهنگ سلولی اولیه، انسان، موش، ایمنی فزاینده،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
چکیده انگلیسی
Intestinal epithelial cells (IECs) serve as an important physiologic barrier between environmental antigens and the host intestinal immune system. Thus, IECs serve as a first line of defense and may act as sentinel cells during inflammatory insults. Despite recent renewed interest in IEC contributions to host immune function, the study of primary IEC has been hindered by lack of a robust culture technique, particularly for small intestinal and adult tissues. Here, a novel adaptation for culture of primary IEC is described for human duodenal organ donor tissue as well as duodenum and colon of adult mice. These epithelial cell cultures display characteristic phenotypes and are of high purity. In addition, the innate immune function of human primary IEC, specifically with regard to Toll-like receptor (TLR) expression and microbial ligand responsiveness, is contrasted with a commonly used intestinal epithelial cell line (HT-29). Specifically, TLR expression at the mRNA level and production of cytokine (IFNγ and TNFα) in response to TLR agonist stimulation is assessed. Differential expression of TLRs as well as innate immune responses to ligand stimulation is observed in human-derived cultures compared to that of HT-29. Thus, use of this adapted method to culture primary epithelial cells from adult human donors and from adult mice will allow for more appropriate studies of IECs as innate immune effectors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 414, 1 December 2014, Pages 20-31
Journal: Journal of Immunological Methods - Volume 414, 1 December 2014, Pages 20-31
نویسندگان
Christina L. Graves, Scott W. Harden, Melissa LaPato, Michael Nelson, Byron Amador, Heather Sorenson, Charles J. Frazier, Shannon M. Wallet,