کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8418179 1545715 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Defining ELISpot cut-offs from unreplicated test and control wells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Defining ELISpot cut-offs from unreplicated test and control wells
چکیده انگلیسی
In the absence of replication of wells, empirical criteria for enzyme-linked immunospot (ELISpot) positivity use fixed differences or ratios between spot forming units (SFU) counts between test and control. We propose an alternative approach which first identifies the optimally variance-stabilizing transformation of the SFU counts, based on the Bland-Altman plot of the test and control wells. The second step is to derive a positivity threshold from the difference in between-plate distribution functions of the transformed test and control SFU counts. This method is illustrated using 1309 assay results from a cohort study of influenza in Vietnam in which some, but not all, of the peptide pools have clear tendencies for SFU counts to be higher in test than control wells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 392, Issues 1–2, 28 June 2013, Pages 57-62
نویسندگان
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