Hyaluronidase treatment of synovial fluid to improve assay precision for biomarker research using multiplex immunoassay platforms

► Synovial fluid is a challenging matrix to analyse. ► Within-subject experimental design to compare treatment methods. ► Hyaluronidase treatment gives better intra-assay precision and signal than diluted or neat samples on the Luminex platform. ► These benefits were inconclusive in MSD assays. ► We recommend hyaluronidase treatment of synovial fluid for osteoarthritis biomarker research.