Assessment of a passive immunity mouse model to quantitatively analyze the impact of neutralizing antibodies on adeno-associated virus-mediated gene transfer

► 69.57% of the rhesus donors had pre-existing anti-AAV8 NAb by assay in vitro. ► 17 rhesus donors (74.0%) had pre-existing anti-AAV8 neutralization by in vivo NAb assay. ► 13.04% of samples had anti-AAV8 NAb titers lower than 1:20 by assay in vitro. ► NAb assay in vivo is superior to assay in vitro for the sample with low level NAb titer.