High-throughput, multiplexed IgG subclassing of antigen-specific antibodies from clinical samples

► We describe the use of Luminex bead arrays to characterize antibodies in clinical samples. ► Using this technology, up to 500 antigen specificities can be assessed at once. ► Simultaneous readouts of antibody subclass can be performed. ► This data provides a comprehensive profile of the antibody response. ► Array signatures can predict antibody functions.