Detection of neutralizing antibodies to erythropoietin by inhibition of rHuEPO-stimulated EGR1 gene expression in the UT-7/EPO cell line

► We measured the expression of selected genes in rHuEPO-stimulated UT-7 cells. ► The EGR1 gene was expressed in response to rHuEPO in UT-7 and UT-7/EPO cells. ► The dose-response of EGR1 expression to rHuEPO was comparable to the proliferative response. ► Normal human sera did not stimulate EGR1 expression in UT-7/EPO cells. ► Patient serum with neutralizing antibodies to rHuEPO inhibited EGR1 expression.