A sensitive high throughput ELISA for human eosinophil peroxidase: A specific assay to quantify eosinophil degranulation from patient-derived sources

► Eosinophil peroxidase (EPX)-based ELISA to assess for human eosinophils. ► EPX-based ELISA displays no cross-reactivity with other human peroxidases. ► EPX-based ELISA, unlike other commercially available assays, is eosinophil-specific. ► This EPX-based ELISA has utility with several diverse patient-derived fluid samples.