Generation of tissue-specific H-2Kd transgenic mice for the study of Kd-restricted malaria epitope-specific CD8+ T-cell responses in vivo

► Four lines of transgenic mice expressing H-2Kd in different tissues are generated. ► A distinct epitope-specific CD8+ T-cell response is induced in H-2Kd transgenic mice. ► Route of immunization influences the magnitude of epitope-specific CD8+ T-cell response.