Detection of antiphospholipid antibodies by automated chemiluminescence assay

► We tested aCL by a new automated system using the chemiluminescence principle. ► Almost all sera from APS patients were also positive for IgG aCl by chemiluminescence. ► Only 22/73 aCL + sera of patients without APS were confirmed by chemiluminescence. ► We identified 20% of patients positive for LA, negative for both aCL and anti-β2-GPI. ► Chemiluminescence assay may represent an useful tool to identify “true” APS patients.