کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8419067 1545732 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Potency determination of recombinant IFN-alpha based on phosphorylated STAT1 using flow cytometry
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Potency determination of recombinant IFN-alpha based on phosphorylated STAT1 using flow cytometry
چکیده انگلیسی
The interferon (IFN) family of cytokines is recognized as a key component of the innate immune response and the first line of defense against viral infection. The usage of the IFN-alpha as a biopharmaceutical has been mainly applied in the treatment of chronic hepatitis C. In the literature it is possible to find a great variety of methods to determine the potency of these cytokines, and many efforts have been made in order to develop practical bioassays to study the biological activity of IFNs. In this technical note, we present a different approach to determine the potency of a recombinant IFN-alpha preparation based on the activation of the signal transducers and activators of transcription 1 (STAT1) using flow cytometry technique. Under the conditions of this study, this new approach proved to be useful and promising to assess the potency of these biopharmaceuticals and may also be used as an important tool in the quality control of such biological products.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 375, Issues 1–2, 31 January 2012, Pages 271-275
نویسندگان
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