Flow cytometric analysis of cytokine expression in short-term allergen-stimulated T cells mirrors the phenotype of proliferating T cells in long-term cultures

► Phenotypes of CD154-expressing T cells mirror long-term cultured T cell phenotypes. ► 8-day cultures show highest statistical power to detect phenotypical differences. ► Phenotypes of enriched T cell lines are similar to those of 8-day cultured PBMCs.