Human kappa light chain targeted Pseudomonas exotoxin A - identifying human antibodies and Fab fragments with favorable characteristics for antibody-drug conjugate development

► A tool for screening of antibodies suitable for antibody-drug conjugate development. ► Exotoxin A was fused to a domain antibody specific for human kappa light chains. ► Antibody candidates were successfully identified using a-kappa-ETA'. ► Candidate antibodies were converted to immunotoxins exerting high cytotoxicity.