A filtration-based protocol to isolate human Plasma Membrane-derived Vesicles and exosomes from blood plasma

► We present a filtration method for isolating Plasma Membrane-derived Vesicles and exosomes from blood. ► In a cohort of 57 healthy donors we found the plasma PMV range was 0.51-2.82 × 105 PMVs/ml. ► Gender and age have no effect on PMV levels whilst fasting increases and smoking decreases levels.