کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8419947 | 1545764 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Perfusion fixation preserves enhanced yellow fluorescent protein and other cellular markers in lymphoid tissues
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
GBCGFPPNAEYFPGCC(4-hydroxy-3-nitrophenyl)acetyl - (4-هیدروکسی-3-نیترفنیل) استیلpeanut agglutinin - آگلوتینین بادام زمینیLymphoid organs - ارگانهای لنفاویtissue sections - بخش های بافتImmunofluorescence microscopy - میکروسکوپ ایمونوفلورسانسenhanced yellow fluorescent protein - پروتئین فلورسنت زرد افزایش یافته استgreen fluorescent protein - پروتئین فلورسنت سبز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Perfusion fixation preserves enhanced yellow fluorescent protein and other cellular markers in lymphoid tissues Perfusion fixation preserves enhanced yellow fluorescent protein and other cellular markers in lymphoid tissues](/preview/png/8419947.png)
چکیده انگلیسی
Fluorescent proteins are increasingly being used to analyze cellular gene expression and to facilitate tracking of cell lineages in vivo. One of these, enhanced yellow fluorescent protein (EYFP) has several properties such as intense fluorescence and little to no toxicity in cells, which makes it an excellent molecule to label proteins and cells of interest. In live cells, visualization of EYFP has been highly successful; however, detection of EYFP in lymphoid tissue sections, particularly in combination with other markers of interest has been difficult. This is because of the enhanced solubility of EYFP in the absence of fixation. When extended fixation protocols are employed, EYFP is preserved but detection of other cellular antigens becomes problematic due to over fixation. Here we demonstrate that EYFP-expressing T and B cells can be efficiently visualized in lymphoid tissue sections without compromising the ability to detect other cellular markers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 340, Issue 2, 30 January 2009, Pages 116-122
Journal: Journal of Immunological Methods - Volume 340, Issue 2, 30 January 2009, Pages 116-122
نویسندگان
Joseph G. Dauner, Craig P. Chappell, Ifor R. Williams, Joshy Jacob,