کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8419986 1545772 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Human recombinant Fab fragments with sub-nanomolar affinities for acetylated histones
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Human recombinant Fab fragments with sub-nanomolar affinities for acetylated histones
چکیده انگلیسی
Acetylation of lysines at different sites in the N-terminus of core histones is a common mode of chromatin modification; different combinations of such modifications are associated with distinct patterns of gene expression, replication and repair. Antibodies are usually used to identify and localize particular histone modifications and to correlate their presence with transcription or other cellular processes. This requires antibodies of sufficient specificity and affinity for each of the many modifications that have now been observed. In most instances, polyclonal antibodies have been used but monoclonal antibodies can also be effective. Here we report that a phage-displayed repertoire of rearranged antibody genes from splenic B cells from a patient with systemic lupus contain Fab fragments that can bind native acetylated lysine 8 histone H4. This finding represents the first selection of human antibodies specific for acetylated histone and suggests that lupus antibodies may contribute to dissection of the histone code.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 329, Issues 1–2, 1 January 2008, Pages 1-10
نویسندگان
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