کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8422928 | 1545964 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Multicellular behavior of environmental Escherichia coli isolates grown under nutrient-poor and low-temperature conditions
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
The multicellular behavior designated “red dry and rough” (rdar) morphotype-characterized by production of extracellular matrix mainly comprising curli fimbriae and cellulose-is a potential survival strategy of Escherichia coli outside the host. This study documents the ability of Escherichia cryptic clades, which have recently been recognized as new lineages genetically divergent from E. coli, to grow in unfavorable conditions through expression of distinct phenotypes. Growth under low-temperature and nutrient-poor conditions induced the rdar morphotype in all cryptic clade strains tested, especially after preincubation in broth supplemented with uracil. Such phenotypic response to harsh growth conditions was clearly detected by transmission and scanning electron microscopy, which showed that bacteria were encased in a fibrous matrix. Conversely, cells incubated in rich medium at 37â¯Â°C showed no matrix. Uracil enhanced the biosynthesis of matrix components, fostering biofilm production and strain adhesion to abiotic surfaces, as demonstrated by the increase of strong biofilm producers in biofilm assays. Harsh growth conditions also induced catalase activity, resulting in clade strain resistance to hydrogen peroxide oxidative stress. The present findings further support the 'environmental hypothesis' whereby cryptic clades would be able to persist in natural habitats outside the host through the expression of distinct survival phenotypes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 210, May 2018, Pages 43-50
Journal: Microbiological Research - Volume 210, May 2018, Pages 43-50
نویسندگان
Laura Di Sante, Armanda Pugnaloni, Francesca Biavasco, Eleonora Giovanetti, Carla Vignaroli,