کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8455499 | 1548023 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential expression of cathepsins K, S and V between young and aged Caucasian women skin epidermis
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کلمات کلیدی
ECMMMPDTTAmCE-64CystatinsNidogen-1Dermal–epidermal junction - اتصال بین پوست و اپیدرمالbenzyloxycarbonyl - بنزیلوسیکروبنیلDEJ - دژdithiothreitol - دیتیوتریتولAging - سالخوردگیExtracellular matrix - ماتریکس خارج سلولیMatrix metalloprotease - ماتریکس متیل پروتئازایCysteine proteases - پروتئینهای سیتینKeratinocytes - کراتینوسیت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cutaneous aging translates drastic structural and functional alterations in the extracellular matrix (ECM). Multiple mechanisms are involved, including changes in protease levels. We investigated the age-related protein expression and activity of cysteine cathepsins and the expression of two endogenous protein inhibitors in young and aged Caucasian women skin epidermis. Immunofluorescence studies indicate that the expression of cathepsins K, S and V, as well as cystatins A and M/E within keratinocytes is reduced in photoprotected skin of aged women. Furthermore, the overall endopeptidase activity of cysteine cathepsins in epidermis lysates decreased with age. Albeit dermal elastic fiber and laminin expression is reduced in aged skin, staining of nidogen-1, a key protein in BM assembly that is sensitive to proteolysis by cysteine, metallo- and serine proteases, has a similar pattern in both young and aged skin. Since cathepsins contribute to the hydrolysis and turnover of ECM/basement membrane components, the abnormal protein degradation and deposition during aging process may be related in part to a decline of lysosomal/endosomal cathepsin K, S and V activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Matrix Biology - Volume 33, January 2014, Pages 41-46
Journal: Matrix Biology - Volume 33, January 2014, Pages 41-46
نویسندگان
Juliette Sage, Delphine De Quéral, Emmanuelle Leblanc-Noblesse, Robin Kurfurst, Sylvianne Schnebert, Eric Perrier, Carine Nizard, Gilles Lalmanach, Fabien Lecaille,