Molecular analysis of point mutations in a barley genome exposed to MNU and gamma rays

► MNU and γ rays revealed 2.2 and 0.3 mutations per 1 Mb of gene sequences. ► The majority (63.6%) of the MNU-induced nucleotide changes were transitions. ► The nucleotide composition surrounding the methylated guanine was checked. ► We obtained higher level of AFLP polymorphism in MNU-treated population. ► The types of identified sequences reflected the organisation of the barley genome.