کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456215 | 1548543 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Extreme testing of undiluted e-cigarette aerosol in vitro using an Ames air-agar-interface technique
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ICR-191UTCNaN32NFNQOAAI2-aminoanthraceneOECD2-nitrofluorene - 2-نیتروفلوئورن4-Nitroquinoline 1-oxide - 4-نیتریکینولین 1-اکسیدB[a]P - B [a] PDMSO - DMSOAAN - ONAli - اماAmes - امزstandard deviation - انحراف معیارBenzo[a]pyrene - بنزو [a] پییرنIn vitro - درونکشتگاهیdimethyl sulphoxide - دی متیل سولفوکسیدair liquid interface - رابط مایع هواOrganisation for Economic Co-operation and Development - سازمان همکاری اقتصادی و توسعهsodium azide - سدیم آیزیدE-cigarette - سیگار الکترونیکیElectronic cigarette - سیگار برقیCooperation Centre for Scientific Research Relative to Tobacco - مرکز همکاری تحقیقات علمی مربوط به دخانیاتICH - منuntreated control - کنترل بدون کنترلInternational Conference on Harmonisation - کنفرانس بین المللی هماهنگیCORESTA - کورستا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
There is a growing consensus that e-cigarettes hold the potential for reducing the harm associated with cigarette smoking. Recently published studies have reported in vitro testing of e-cigarettes, demonstrating reduced toxicological and biological effects. Few studies however have reported the use of e-cigarettes under extreme testing conditions. To assess the full mutagenic potential of a commercially available electronic-cigarette (Vype ePen), this study investigated the delivery of aerosol under extreme conditions, using a scaled-down 35â¯mm plate Ames bacterial reverse mutagenicity assay. S. typhimurium strains TA98, TA100, TA97, TA104 and E. coli WP2 uvrA pKM101 with or without metabolic activation (S9), were employed. Using a modified Vitrocell VC 10 exposure system 0, 180, 360, 540, 720 or 900 puffs of undiluted e-cigarette aerosol was generated and delivered to bacterial cultures aligned to reported human consumption data. The results demonstrate that no mutagenic activity was observed in any strain under any test condition even when exposed to 900 puffs of undiluted e-cigarette aerosols +/â S9. Positive control responses were observed in all strainsâ¯+/â S9. Nicotine assessments demonstrated an increased and consistent aerosol delivery, with calculated maximum doses of â¼1â¯mg/mL delivery of nicotine. These data demonstrate the validity of this unique testing approach and adds further information to the growing weight of evidence that e-cigarettes offer substantially reduced exposure when compared to conventional cigarette smoke. For future in vitro assessments of next generation tobacco and nicotine products, the generation, delivery and testing of undiluted aerosols can now be considered.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 828, April 2018, Pages 46-54
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 828, April 2018, Pages 46-54
نویسندگان
D. Thorne, M. Hollings, A. Seymour, J. Adamson, A. Dalrymple, M. Ballantyne, M. Gaca,