کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456249 | 1548547 | 2017 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Generation of Advanced Glycation End-Products (AGEs) by glycoxidation mediated by copper and ROS in a human serum albumin (HSA) model peptide: reaction mechanism and damage in motor neuron cells
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کلمات کلیدی
3-deoxyglucosoneAverage roughnessGlycoxidationCytokinesis-blocked micronucleus assayHPLC-ESI-MSNBUDNPBCBMNUMPAGEsAFM3-DGCMLHSAROS - ROShuman serum albumin - آلبومین سرم انسانیnuclear buds - جوانه های هسته ایDiabetes mellitus - دیابت قندیmicronuclei - ریزهستک یا میکرونوکلئوس Genotoxicity - سمیت ژنتیکیadvanced glycation end-products - محصولات نهایی گلیسایی پیشرفتهCopper - مسatomic force microscopy - میکروسکوپ نیروی اتمیCarboxymethyl lysine - کربوکسی متیل لیزینGlucose - گلوکزReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glucose, in the presence of reactive oxygen species (ROS), acts as an as an oxidative agent and drives deleterious processes in Diabetes Mellitus. We have studied the mechanism and the toxicological effects of glucose-dependent glycoxidation reactions driven by copper and ROS, using a model peptide based on the exposed sequence of Human Serum Albumin (HSA) and containing a lysine residue susceptible to copper complexation. The main products of these reactions are Advanced Glycation End-products (AGEs). Carboxymethyl lysine and pyrraline condensed on the model peptide, generating a Modified Peptide (MP). These products were isolated, purified, and tested on cultured motor neuron cells. We observed DNA damage, enhancement of membrane roughness, and formation of domes. We evaluated nuclear abnormalities by the cytokinesis-blocked micronucleus assay and we measured cytostatic and cytotoxic effects, chromosomal breakage, nuclear abnormalities, and cell death. AGEs formed by glycoxidation caused large micronucleus aberrations, apoptosis, and large-scale nuclear abnormalities, even at low concentrations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 824, December 2017, Pages 42-51
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 824, December 2017, Pages 42-51
نویسندگان
Caroline Martins Sandanielo Marques, Emilene Arusievicz Nunes, Larissa Lago, Cibele Nicolaski Pedron, Tânia Maria Manieri, Roseli Hiromi Sato, Vani Xavier Junior, Giselle Cerchiaro,