کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456421 | 1548582 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Repeated dose liver and gastrointestinal tract micronucleus assays using N-methyl-Nâ²-nitro-N-nitrosoguanidine in young adult rats
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Repeated dose liver and gastrointestinal tract micronucleus assays using N-methyl-Nâ²-nitro-N-nitrosoguanidine in young adult rats Repeated dose liver and gastrointestinal tract micronucleus assays using N-methyl-Nâ²-nitro-N-nitrosoguanidine in young adult rats](/preview/png/8456421.png)
چکیده انگلیسی
N-Methyl-Nâ²-nitro-N-nitrosoguanidine (MNNG) is a direct-acting mutagen that induces tumors in the glandular stomach, but not in the liver or colon, of rats after oral administration. To evaluate the performance of repeated dose liver and gastrointestinal tract micronucleus (MN) assays in young adult rats, MNNG was administered by oral gavage to male CD (SD) rats aged 6 weeks at doses of 0 (vehicle; 2.5% DMSO aqueous solution), 3.125, 6.25, 12.5, and 25Â mg/kg/day once daily for 14 and 28 days, and the MN frequencies were examined in the hepatocytes, glandular stomach cells, and colonic cells. The MN induction in immature erythrocytes in the bone marrow of these animals was also simultaneously evaluated. The frequencies of micronucleated (MNed) glandular stomach cells were significantly increased in all MNNG treatment groups in a dose-dependent manner in both repeated dose studies. In contrast, the frequencies of MNed hepatocytes and colonic cells were not significantly increased compared to the vehicle control. In the bone marrow, a small but significant increase in the frequency of MNed immature erythrocytes was observed only at the highest dose in the 28-day study. Since a clear positive result in the glandular stomach agrees with the tissue specificity of tumor induction by this chemical, the MN assay with the glandular stomach, which is a direct contact site with high concentrations of test substances administered by oral gavage, may be useful for detecting genotoxic compounds that are short-lived in vivo, such as MNNG.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volumes 780â781, March 2015, Pages 100-106
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volumes 780â781, March 2015, Pages 100-106
نویسندگان
Tomomi Takayanagi, Yumi Wako, Kazufumi Kawasako, Hisako Hori, Wataru Fujii, Wakako Ohyama,