کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456841 | 1548779 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic Characterization of Prostate Cancer to Distinguish Nonmetastasizing and Metastasizing Primary Tumors and Lymph Node Metastases
ترجمه فارسی عنوان
تشخیص پروتئومیکای سرطان پروستات به منظور تشخیص تومورهای اولیه غیر متاستاز و متاستاز و متاستازهای گره لنفاوی
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کلمات کلیدی
RPELNDLNMFFPE - MEPPCA - PCAImmunohistochemistry - ایمونوهیستوشیمیIHC - ایمونوهیستوشیمیLymph node dissection - تشخیص گره لنفاویfold change - تغییر در برابرPrimary tumor - تومور اولیهRadical prostatectomy - رادیکال پروستاتکتومیProstate cancer - سرطان پروستاتMass-spectrometry - طیف سنجی تودهformalin-fixed, paraffin-embedded - فرمالین ثابت، پارافین تعبیه شده استLymph node metastasis - متاستاز غدد لنفاوی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
چکیده انگلیسی
Patients with metastatic prostate cancer (PCa) have a poorer prognosis than patients with organ-confined tumors. We strove to uncover the proteome signature of primary PCa and associated lymph node metastases (LNMs) in order to identify proteins that may indicate or potentially promote metastases formation. We performed a proteomic comparative profiling of PCa tissue from radical prostatectomy (RPE) of patients without nodal metastases or relapse at the time of surgical resection (n = 5) to PCa tissue from RPE of patients who suffered from nodal relapse (n = 5). For the latter group, we also included patient-matched tissue of the nodal metastases. All samples were formalin fixed and paraffin embedded. We identified and quantified more than 1200 proteins by liquid chromatography tandem mass spectrometry with subsequent label-free quantification. An increase of ribosomal or proteasomal proteins in LNM (compared to corresponding PCa) became apparent, while extracellular matrix components rather decreased. Immunohistochemistry (IHC) corroborated accumulation of poly-(ADP-ribose)-polymerase 1 and N-myc-downstream-regulated-gene 3, alpha/beta hydrolase domain-containing protein 11, and protein phosphatase slingshot homolog 3 in LNM. These findings strengthen the present interest in examining PARP inhibitors for the treatment of aggressive PCa. IHC also corroborated increased abundance of retinol dehydrogenase 11 in metastasized primary PCa compared to organ-confined PCa. Generally, metastasizing primary tumors were characterized by an enrichment of proteins involved in cellular lipid metabolic processes with concomitant decrease of cell adhesion proteins. This study highlights the usefulness of a combined proteomic-IHC approach to explore novel aspects in tumor biology. Our initial results open novel opportunities for follow-up studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neoplasia - Volume 20, Issue 2, February 2018, Pages 140-151
Journal: Neoplasia - Volume 20, Issue 2, February 2018, Pages 140-151
نویسندگان
Ann-Kathrin Müller, Melanie Föll, Bianca Heckelmann, Selina Kiefer, Martin Werner, Oliver Schilling, Martin L. Biniossek, Cordula Annette Jilg, Vanessa Drendel,