کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8460444 | 1548983 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mapping the secretome of human chondrogenic progenitor cells with mass spectrometry
ترجمه فارسی عنوان
نقشه برداری مخلوطی از سلولهای پیش گیاه شناسانه انسان با طیف سنجی جرمی
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کلمات کلیدی
آرتروز، مخفیگاه سلول پیشرونده هورمونیک، طیف سنجی جرمی، مهندسی بافت،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
چکیده انگلیسی
Tissue engineering offers promising perspectives in the therapy of osteoarthritis. In the context of cell-based therapy, chondrogenic progenitor cells (CPCs) may be used to regenerate defects in cartilage tissue. An in-depth characterization of the secretome of CPCs is a prerequisite to this approach. In this study, a method was developed for the qualitative and quantitative analysis of the secretome of undifferentiated and differentiated CPCs. Secreted proteins from cells grown in two-dimensional as well as three-dimensional alginate cultures were extracted and analyzed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Quantitation was achieved by internal standardization using stable isotope-labeled amino acids in cell culture (SILAC). Qualitative analysis of CPC secretomes revealed ECM-components, signal proteins and growth factors most of which were also found in healthy cartilage. A quantitative comparison revealed significantly upregulated proteins with regenerative potential during differentiation, while proteins involved in catabolic metabolism were significantly downregulated. The development of methods for qualitative and quantitative analysis of the secretome of CPCs by mass spectrometry provides a foundation for the investigation of progenitor or stem cells from other sources.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 212, July 2017, Pages 4-10
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 212, July 2017, Pages 4-10
نویسندگان
Sarah Batschkus, Ilian Atanassov, Christof Lenz, Philipp Meyer-Marcotty, Gökhan Cingöz, Christian Kirschneck, Henning Urlaub, Nicolai Miosge,