کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8462836 | 1549041 | 2008 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of MUC16 by inflammatory mediators in ocular surface epithelial cell lines
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Regulation of MUC16 by inflammatory mediators in ocular surface epithelial cell lines Regulation of MUC16 by inflammatory mediators in ocular surface epithelial cell lines](/preview/png/8462836.png)
چکیده انگلیسی
The aim of the present study was to evaluate the regulation of membrane-anchored mucin MUC16 by proinflammatory cytokines and bacterial components at the ocular surface. Expression and distribution of MUC16 in conjunctival (HCjE) and corneal (HCE) epithelial cell lines was monitored by RT-PCR and immunohistochemistry. To determine the regulation of MUC16, cultured HCjEs and HCEs were stimulated with different cytokines, bacterial components and bacterial supernatants, and analyzed by real-time PCR, immunodot blot and immunohistochemistry. The results indicate that MUC16 is differentially regulated between HCjEs and HCEs after challenge with inflammatory mediators and suggest shedding of MUC16 from the ocular surface epithelia into the tear film. This seems to be precisely regulated. MUC16 shedding can be differentially increased and decreased, suggesting a protective function of membrane-anchored MUC16 and supporting the hypothesis that dysregulation of membrane-anchored MUC16 at the ocular surface may be involved in dry eye pathology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 190, Issue 1, 28 February 2008, Pages 59-70
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 190, Issue 1, 28 February 2008, Pages 59-70
نویسندگان
Friedrich Paulsen, Kristin Jäger, Dieter Worlitzsch, Lars Bräuer, Ute Schulze, Gesa Schäfer, Saadettin Sel,