Nucleofection of a DNA vaccine into human monocyte-derived dendritic cells

► We use a nucleofection technique for delivering DNA vaccines into hMoDCs. ► We examine the impact of this delivery method on hMoDCs. ► The nucleofection promotes the activation and maturation of hMoDCs. ► Welfare effects depend on dosage of the plasmid and its retention time. ► Non-specific changes happen during the electroporation.