The source of human mesenchymal stromal cells influences their TLR profile as well as their functional properties

► MSC source influences their TLR and cytokine expression profile and their functional properties. ► WJ-MSC do not express TLR4 and express a nonfunctional TLR3. ► Inflammation upregulates TLR3 in all three MSC types and TLR4 only in BM-MSC. ► TLR ligation increases the production of inflammatory cytokines in AT and BM-MSC but not in WJ-MSC. ► The immunosuppressive potential of WJ-MSC was affected neither by inflammation nor by TLR triggering.