کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8464645 1549446 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tolerance of human embryonic stem cell derived islet progenitor cells to vitrification-relevant solutions
ترجمه فارسی عنوان
تحمل سلول های پیش گیاه شناس سلول های بنیادی جنینی انسان به محلول های مرتبط با انجماد
کلمات کلیدی
فریزر تبخیر کردن، تحمل اسمزی، سمی بودن دیابت، پیشگامان انسولین، کپسول مکانیکی، سلولهای بتا،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
We have previously shown that human embryonic stem cell derived islet progenitors (hESC-IPs), encapsulated inside an immunoprotective device, mature in vivo and ameliorate diabetes in mice. The ability to cryopreserve hESC-IPs preloaded in these devices would enhance consistency and portability, but traditional 'slow freezing' methods did not work well for cells encapsulated in the device. Vitrification is an attractive alternative cryopreservation approach. To assess the tolerance of hESC-IPs to vitrification relevant conditions, we here are reporting cell survival following excursions in tonicity, exposure to fifteen 40% v/v combinations of 4 cryoprotectants, and varied methods for addition and elution. We find that 78% survival is achieved using a protocol in which cells are abruptly (in one step) exposed to a solution containing 10% v/v each dimethyl sulfoxide, propylene glycol, ethylene glycol, and glycerol on ice, and eluted step-wise with DPBS + 0.5 M sucrose at 37 °C. Importantly, the hESC-IPs also maintain expression of the critical islet progenitor markers PDX-1, NKX6.1, NGN3 and NEURO-D1. Thus, hESC-IPs exhibit robust tolerance to exposure to vitrification solutions in relevant conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 70, Issue 3, June 2015, Pages 283-286
نویسندگان
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