کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8470034 | 1549706 | 2012 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
δ-Aminolevulinate synthase is required for apical transcellular barrier formation in the skin of the Drosophila larva
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: δ-Aminolevulinate synthase is required for apical transcellular barrier formation in the skin of the Drosophila larva δ-Aminolevulinate synthase is required for apical transcellular barrier formation in the skin of the Drosophila larva](/preview/png/8470034.png)
چکیده انگلیسی
Animals construct a layered skin to prevent dehydration and pathogen entrance. The barrier function of the skin relies on the extensive cross-linking of specialised components. In insects, for instance, epidermal cells produce an apical extracellular cuticle that consists of a network of proteins, chitin and lipids. We have identified mutations in the Drosophila gene coding for the δ-aminolevulinate synthase (Alas) that cause massive water loss. The cuticle of alas mutant larvae detaches from the epidermis and its basal region is frayed suggesting that an Alas dependent pathway is needed to organise the contact between the cuticle and the epidermis and anchor the cuticle to the apical surface of epidermal cells. Concomitantly, reduction of Alas function results in weakening of the extracellular dityrosines network in the cuticle, whereas glutamyl-lysine isopeptide bonds are not affected. The lateral septate junctions of epidermal cells that serve as a paracellular plug are intact, as well. Taken together, we hypothesise that Alas activity, which initiates heme biosynthesis in the mitochondrion, is needed for the formation of a dityrosine-based barrier that confers resistance to the internal hydrostatic pressure protecting both the cuticle from transcellular infiltration of body fluid and the animal from dehydration. We conclude that at least two modules - an apical protein-chitin lattice and the lateral septate junctions, act in parallel to ensure Drosophila skin impermeability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 91, Issue 3, March 2012, Pages 204-215
Journal: European Journal of Cell Biology - Volume 91, Issue 3, March 2012, Pages 204-215
نویسندگان
Khaleelulla Saheb Shaik, Frauke Meyer, Ángel Vizoso Vázquez, Matthias Flötenmeyer, Maria Esperanza Cerdán, Bernard Moussian,