کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8470498 1549997 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Spiral-based microfluidic device for long-term time course imaging of Neurospora crassa with single nucleus resolution
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Spiral-based microfluidic device for long-term time course imaging of Neurospora crassa with single nucleus resolution
چکیده انگلیسی
Real-time imaging of fluorescent reporters plays a critical role in elucidating fundamental molecular mechanisms including circadian rhythms in the model filamentous fungus, Neurospora crassa. However, monitoring N. crassa for an extended period of time with single nucleus resolution is a technically challenging task due to hyphal growth that rapidly moves beyond a region of interest during microscopy experiments. In this report, we have proposed a two-dimensional spiral-based microfluidic platform and applied for monitoring the single-nucleus dynamics in N. crassa for long-term time course experiments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 94, September 2016, Pages 11-14
نویسندگان
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