کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8474399 | 1550425 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mobilization of endothelial progenitor cells in acute cardiovascular events in the PROCELL study: Time-course after acute myocardial infarction and stroke
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کلمات کلیدی
CECsVCAMSTEMIvWFTIANSTEMIEPCsNIHSSAmI - AMIMyocardial infarction - آنفارکتوس میوکاردST-segment elevation myocardial infarction - انفارکتوس میوکارد بالایی قسمت STtransient ischemic attack - حمله ایسکمی گذراEndothelial progenitor cells - سلول های پیش ساز اندوتلیالEndothelial progenitor cell - سلول پیش ساز اندوتلیالCirculating endothelial cells - سلولهای اندوتلیال تومورAcute myocardial infarction - سکته قلبیStroke - سکته مغزیvon Willebrand factor. - عامل فون ویلبراند.Vascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)National Institutes of Health stroke scale - مؤسسات ملی بهداشت در مقیاس سکته مغزیCell-adhesion molecule - مولکول چسبندگی سلولیvascular cell adhesion molecule - مولکول چسبندگی سلولی عروقی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The mobilization pattern and functionality of endothelial progenitor cells after an acute ischemic event remain largely unknown. The aim of our study was to characterize and compare the short- and long-term mobilization of endothelial progenitor cells and circulating endothelial cells after acute myocardial infarction or atherothrombotic stroke, and to determine the relationship between these cell counts and plasma concentrations of vascular cell adhesion molecule (VCAM-1) and Von Willebrand factor (VWF) as surrogate markers of endothelial damage and inflammation. In addition, we assessed whether endothelial progenitor cells behave like functional endothelial cells. We included 150 patients with acute myocardial infarction or atherothrombotic stroke and 145 controls. Endothelial progenitor cells [CD45 â, CD34 +, KDR +, CD133 +], circulating endothelial cells [CD45 â, CD146 +, CD31 +], VWF, and VCAM-1 levels were measured in controls (baseline only) and in patients within 24 h (baseline) and at 7, 30, and 180 days after the event. Myocardial infarction patients had higher counts of endothelial progenitor cells and circulating endothelial cells than the controls (201.0/mL vs. 57.0/mL; p < 0.01 and 181.0/mL vs. 62.0/mL; p < 0.01). Endothelial progenitor cells peaked at 30 days post-infarction (201.0/mL vs. 369.5/mL; p < 0.01), as did VCAM-1 (573.7 ng/mL vs. 701.8 ng/mL; p < 0.01). At 180 days post-infarction, circulating endothelial cells and VWF decreased, compared to baseline. In stroke patients, the number of endothelial progenitor cells - but not circulating endothelial cells - was higher than in controls (90.0/mL vs. 37.0/mL; p = 0.01; 105.0/mL vs. 71.0/mL; p = 0.11). At 30 days after stroke, however, VCAM-1 peaked (628.1/mL vs. 869.1/mL; p < 0.01) but there was no significant change in endothelial progenitor cells (90/mL vs. 78/mL; p < 0.34). At 180 days after stroke, circulating endothelial cells and VWF decreased, compared to baseline. Cultured endothelial progenitor cells from controls and myocardial infarction patients had endothelial phenotype characteristics and exhibited functional differences in adhesion and Ca2 + influx, but not in proliferation and vasculogenesis. In myocardial infarction patients, VCAM-1 levels and mobilization of endothelial progenitor cells peaked at 30 days after the ischemic event. Although a similar VCAM-1 kinetic was observed in stroke patients, endothelial progenitor cells did not increase. Endothelial progenitor cells had mature endothelial capabilities in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 80, March 2015, Pages 146-155
Journal: Journal of Molecular and Cellular Cardiology - Volume 80, March 2015, Pages 146-155
نویسندگان
Ander Regueiro, Elisa Cuadrado-Godia, Carlos Bueno-BetÃ, Maribel Diaz-Ricart, Anna Oliveras, Susana Novella, Gemma González Gené, Carole Jung, Isaac Subirana, Jose Tomás Ortiz-Pérez, Mercè Roqué, Xavier Freixa, Julio Núñez, Gines Escolar,