Superresolution microscopy in heart - Cardiac nanoscopy

► Conventional fluorescence microscopy is limited in resolution by diffraction. ► Rapidly evolving nanoscale imaging techniques overcome the resolution barrier. ► We discuss recent cardiac applications of superresolution techniques. ► Targeted readout versus stochastic readout are major superresolution principles. ► Subcellular Ca2 + signaling nanodomains and organelles are highlights of nanoscopy.