Overexpression of AMP-activated protein kinase or protein kinase D prevents lipid-induced insulin resistance in cardiomyocytes

► Overexpression of AMPK and PKD prevents loss of insulin-stimulated glucose uptake. ► AMPK overexpression prevents lipid-induced loss of insulin-signalling. ► PKD overexpression prevents triacylglycerol accumulation during lipid oversupply. ► Both AMPK and PKD prevent lipid-induced loss of insulin-stimulated glucose uptake.