کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8476329 | 1550805 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adrenergic receptor stimulation suppresses oxidative metabolism in isolated rat islets and Min6 cells
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کلمات کلیدی
(HBSS)(GO)Krebs Ringer buffer(CA)G protein coupled receptor - G پروتئین همراه با پروتئینadenylyl cyclase - آدنیلات سیکلاز، آدنیلیل سیکلازtetramethylrhodamine ethyl ester perchlorate - تترامتیل رودامین اتیل استر پرکلراتInsulin secretion - ترشح انسولینPancreatic islet - جزیره پانکراسelectron transport chain - زنجیره انتقال الکترونOxidative phosphorylation - فسفوریلاسیون اکسیداتیوOxygen consumption rate - میزان مصرف اکسیژنGene ontology - هستیشناسی ژنیProteomics - پروتئومیکسcatecholamine - کاتکولآمینهاGlucose stimulated insulin secretion - گلوکز ترشح انسولین را تحریک می کندadrenergic receptor - گیرنده آدرنرژیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
Insulin secretion is stimulated by glucose metabolism and inhibited by catecholamines through adrenergic receptor stimulation. We determined whether catecholamines suppress oxidative metabolism in β-cells through adrenergic receptors. In Min6 cells and isolated rat islets, epinephrine decreased oxygen consumption rates compared to vehicle control or co-administration of epinephrine with α2-adrenergic receptor antagonist yohimbine. Epinephrine also decreased forskolin-stimulated oxygen consumption rates, indicating cAMP dependent and independent actions. Furthermore, glucose oxidation rates were decreased with epinephrine, independent of the exocytosis of insulin, which was blocked with yohimbine. We evaluated metabolic targets through proteomic analysis after 4â¯h epinephrine exposure that revealed 466 differentially expressed proteins that were significantly enriched for processes including oxidative metabolism, protein turnover, exocytosis, and cell proliferation. These results demonstrate that acute α2-adrenergic stimulation suppresses glucose oxidation in β-cells independent of nutrient availability and insulin exocytosis, while cAMP concentrations are elevated. Proteomics and immunoblots revealed changes in electron transport chain proteins that were correlated with lower metabolic reducing equivalents, intracellular ATP concentrations, and altered mitochondrial membrane potential implicating a new role for adrenergic control of mitochondrial function and ultimately insulin secretion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 473, 15 September 2018, Pages 136-145
Journal: Molecular and Cellular Endocrinology - Volume 473, 15 September 2018, Pages 136-145
نویسندگان
Amy C. Kelly, Leticia E. Camacho, Ken Pendarvis, Hailey M. Davenport, Nathan R. Steffens, Kate E. Smith, Craig S. Weber, Ronald M. Lynch, Klearchos K. Papas, Sean W. Limesand,